The Logan Group

 

Dr. Nilakshee Bhattacharya, Postdoctoral Fellow


Nilakshee is investigating the DNA binding by IdeR. She measures binding as a function of promoter sequence and metal concentration using, typically, electrophoretic mobility shift assays.
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Jason Crowe, Molecular Biophysics Graduate Student


Jason is pioneering a new project using NMR spectroscopy to study stem cell metabolism. He has designed a very clever three dimensional bioreactor that will allow us to use 31P and possibly 13C NMR spectroscopy to study metabolism of these cells in vivo.
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Josh Kogot, Biochemistry Graduate Student


Josh is co-mentored by Dr. Logan and Dr. Geoff Strouse. Josh's research focuses on the use of gold nano-particles and NSET spectroscopy in solving biological problems. NSET is an improvement on fluorescence resonance energy transfer spectroscopy in which the distance dependence is extended (out to 200 A) and the dependence on Donor - Acceptor overlap integral and orientation are eliminated. We plan to use this spectroscopy to investigate assembly of protein-DNA complexes, in particular of IdeR.
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Josh graduated in Fall, 2008, and is a postdoc at Army Research Labs
 
 
 

Kyle Noble, Molecular Biophysics Graduate Student


Kyle joined the lab in Spring of 2006. He is working studying the structural and biophysical properties of Delta, a protein that is involved in the notch signalling pathway.
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Dave Skelton, Chemistry and Biochemistry Graduate Student


Dave joined the lab in the Spring, 2007. He is using GC-MS to study metabolism in CHO cells and in stem cells.
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Brian Stapleton, Chemistry and Biochemistry Graduate Student


Brian joined the lab in the Spring, 2007. Brian is studying metal binding in IdeR, comparing the binding energetics of various metals. He is also investigating the linkage between metal binding and dimerization using analytical ultracentrifugation.
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Larry Walker, Chemistry and Biochemistry Graduate Student


Larry joined the lab in the Spring, 2007. Larry is investigating the molecular basis for "hyperactivity" in a mutant of IdeR. This protein exhibits functional repressor activity under low metal concentrations where the wildtype repressor is no longer active. We are interested in this protein because its over-expression in virulent Mtb can reduce virulence.
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Nathalie Munoz, Molecular Biophysics Graduate Student


Nathalie joined my lab in Spring, 2009. She will continue the collaboration with Dr. Geoff Strouse, using nanoparticles and quantum dots for delivering genes and other biologically important cargo to cells.
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Abbey Goodyear, Biochemistry Undergraduate Student


Abbey has worked in my lab for the past year. Her research involves monitoring the activity of enzymes and signalling pathways controlling metabolism. She is currently working on our CHO cells, but will also work with the stem cells.
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Former Logan Group Members

 

Wendy Walton, Ph.D, Molecular Biophysics, December, 2005


Wendy defended her dissertation on December 9, 2005. Her doctoral research was devoted to optimizing the production and purification of recombinant Thy-1 glycoprotein in Lec1 mammalian and Sf9 or Tn5 insect cells. She has optimized culture procedures, purification protocols, and has worked to identify an economical approach to isotope enrichment of recombinant proteins produced in Lec1 cells. Wendy has accepted a position at Schering-Plough and started at the end of May, 2006. Good luck, Wendy!

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K. Ilker Sen, Ph.D, Molecular Biophysics, January, 2007


Ilker (that's him on the right) used EPR spectroscopy to investigate the activation of the anthracis repressor (AntR), the DtxR homologue from Bacillus anthracis. He has used Mn(II) EPR spectroscopy to determine metal binding energetics and to characterize the nature of the metal binding site. More recently he has been refining methods for determining inter-spin distances via the 4-pulse DEER experiment. He is applying these methods to monitor conformational changes in AntR as a function of metal binding. Ilker defended his dissertation in January, 2007, and is now a postdoc at NYU. Good Luck, Ilker!

 

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Maria Semavina, Ph.D, Molecular Biophysics, April, 2006


Maria investigated the energetics of metal binding and dimerization during the activation of DtxR and IdeR. She has used equilibrium dialysis to determine metal binding energetics. She also used analytical ultracentrifugation to determine dimerization energetics as a function of metal ligation in IdeR. These data are being analyzed to suggest a mechanism for the activation of DtxR and IdeR upon metal binding. Maria is currently a postdoc at Cornel Medical Center. Good Luck, Maria!

 

 

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Sumiko Takahashi, M.S., Chemistry and Biochemistry, May, 2006


Sumiko defended her Master's Thesis in the Spring, 2006. After travelling around for a few months she returned to work in my lab before obtaining a permanent position at Pfizer in Nagoya, Japan, which she started in March, 2007. Good luck, Sumiko!

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Fun Logan Group Pictures

Nilakshee and Sumiko in Traditional Dresses.
Tim and Nancy skiing in Colorado.
 
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