Protein Crystallization and X-Ray Diffraction (pcxrd)

MOB August Boot camp

The practical course in crystallization and diffraction is intended to be a precursor to the one semester graduate level macromolecular crystallization special topics course taught by 9-month faculty every other year. In this course, we will cover basic concepts of crystals, symmetry, crystallization, x-ray generation, radiation safety, x-ray diffraction, cryo safety, cryogenic technique, data collection, data processing, Linux o/s, computer graphics, data visualization, and model building. More emphasis will be given to hands-on training and lab work than the full-fledged graduate course.

At the end of this course, you will learn basic concepts in:

Protein crystallization and crystal symmetry Cryogenic safety and cryo-crystallography
Need for crystals, cryogenic temperatures, and X-Rays X-Ray generation and safety
X-Ray diffraction and data collection Linux operating system and running programs
Data Processing and analysis Computer graphics and model visualization
2017 Course Schedule
 Duration of the program: 3 weeks Number of days for experiments: 6
 Flexible for expansion of experiments and data  Experience in wet lab, instrumentation, and computation
Course Syllabus [Detailed Syllabus]

1.  Symmetry, lattices, crystals, and crystallization
2. Crystals and protein crystallization set-up
3. Crystallization observation and optimization
4. X-Rays, radiation safety and X-Ray generation
5. Cryogenics safety and cryo-crystallography
6. X-Ray Diffraction set-up and data collection
7. Linux operating system and computer graphics
8. Data processing and visualization and wrap-up

Sessions high-lighted in bold will involve more hands-on training time and sessions high-lighted in italics will involve more lecture time.

Core Facility Contacts

Computer Resources Facility
Director: Dr. Michael Zawrotny
Phone: 850-644-0069
Maillist: IMBPeople
Location: KLB 415

Physical Biochemistry Facility
Director: Dr. Claudius Mundoma
Phone: 850-644-5147
Location: KLB 311-318
Maillist: PBFusers

Protein Expression Facility
Director: Dr. Joan Hare
Phone: 850-644-1421
Location: KLB 312 Suite
Maillist: PEFusers

X-Ray Facility
Director: Dr. T. "Soma"sundaram
Phone: 850-644-6448
Location: KLB 410-413
Maillist: Xrayusers
URL: biophysics.fsu.edu/soma/x-ray-facility/

Common Equipment Labs
Electronics--Contact: Rick Wang 850-645-3309
Machine Shop & Building Maintenance--Contact: Wade Baggett 850-644-1419