Insect Flight Muscle

still awaiting

Portion of an electron tomogram assembled from rebuilt class averages of isometrically contracting insect flight muscle. To make this picture, the thick filaments were segmented separately from the aligned raw repeats and then placed back into an idealized lattice with appropriate rotations and translations. A column average was then computed. To obtain the thin filaments and their crossbridges, a reassembled class average of each original raw repeat was placed into an idealized lattice with appropriate rotations and translations and then low pass filtered to the original resolution of the tomogram. The two maps were then displayed in Chimera, the column average of thick filaments colored yellow and the reassembled thin filaments colored gray. Myosin head attachments to the troponin complex were subsequently colored green in Adobe Photoshop.

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