Electron Transfer in Sulfite Reductase
Sulfite Reductase (SiR) is responsible for the six-electron reduction of sulfite to sulfide, a six-electron reduction. It is composed of two subunits, a hemoprotein and a flavoprotein, that work together with their resident cofactors to move electrons from the cytoplasm to the substrate. We aim to understand how the proteins work with their cofactors to perform this rich, complex chemical transformation.
The Molecular Basis of Gene Expression
Genetic information comes to life when a DNA molecule is transcribed into a messenger RNA molecule before being translated into protein. In eukaryotes, special sequences of DNA help control how and when genes are transcribed. Once in the cytoplasm, resulting mRNA molecules are translated into proteins by the ribosome. The goal of our research is to understand the structural basis for regulating non-Watson-Crick DNA structures called guanine quadruplexes that help control the flow of genetic information in the nucleus. We are also studying how the small subunit of the the ribosome is assembled, focusing on understanding how structural heterogeneity affects function.
The lab uses a combination of x-ray crystallography and cryo-EM to determine the structure and function of these important biomolecules.
FSU’s Biological Science Imaging Resource is the home of a state-of-the art cryo-TEM called the Titan Krios, one of only a few in the world.