The multi-user macromolecular x-ray crystallography facility (XRF) at the Kasha Laboratory Building (KLB) in the Institute of Molecular Biophysics (IMB) provides the instrument and expertise for collecting and processing x-ray diffraction data from macromolecular single crystals. It is one of the Core Facilities available to all the members of Structural Biology Program and members of FSU. XRF is located on the east side of the fourth floor of KLB (see the floor plan). More facility information is available at XRF Homepage. IMB X-Ray Facility also coordinates additional data collection at the third generation synchrotron x-ray source using FSU’s membership in SER-CAT Beamline 22 in Advanced Photon Source in Argonne National Laboratory, Argonne, IL. XRF also offers Protein Crystal Screening Service to users.
Manuals and Protocols
The following is a short list of manuals, standard operating procedures, and protocols for instruments and equipment at the XRF
- Standard Operating Procedure for Rigaku RU-H2R and RU-H3R X-Ray Generators
- Flow-chart for starting-up and shutting-down Rigaku RU-H3R generator w/ R-Axis IV++ detector and Oxford Cryosystems CryoCooler 700
- Hardware Manuals
- Software Manuals [some need updates; coming soon!]
- Software & Hardware tips [some need updates; coming soon!]
- ARI Crystal Gryphon at FSU Standard Operating Procedure [PDF]
- Making and modifying Gryphon Protocol [PDF]
Instruments and Accessories
XRF is equipped with one each of Rigaku RU-H2R & RU-H3R x-ray generators, two Osmic confocal mirror systems, one Rigaku R-Axis IV++ IP detector and a Mar CCD165 detector. The Facility has two Oxford CryoStream cryocoolers and one American Magnetics auto-refill system. The facility has stereo-zoom microscopes, crystallization chambers maintained at variety of temperatures, and data processing hardware and software. The Facility Director also coordinates data collection work using FSU’s membership in SER-CAT in Advanced Photon Source Beam-line 22. In August 2014 the Facility installed an Art Robbins Instruments Crystal Gryphon for protein crystallization set-up. In October 2014 we have added ThorLabs DCC1645C CMOS USB 2.0 digital camera to the existing Leica S8 APO light microscope.
From January 2016, we started to offer automated scanning and storing of images of 96-reservoir-3-well or 96-reservoir-2-well ARI Intelliplate crystallization screens using our IMB-designed home-built Simple Robotic Imager (SRI). The robotic imager is housed in KLB 412 and is shown on the right collecting data on an ARI 102-0001-20 using a Linux computer. For ARI 96-reservoir-2 well plate (ARI# 102-000-20) it will take about 8 minutes to image all the 192 wells at about 90x magnification. For ARI 96-reservoir-3 well plate (either ARI# 102-000-13 or ARI# 102-000-03 ) it will take about 12 minutes to image all the 288 wells at about 120x magnification. As soon as the image collection is over it can be viewed using Linux feh image viewer program.
In July 2018, XRF acquired Rock Imager 01 manufactured by Formulatrix that is compatible with SBS, 96-well (ARI), and Linbro plates. Rock Imager allows the automated imaging of plates and works with Rock Maker software. Rock Imager 01 has a single light path and has both visible and ultra-violet (UV) light capabilities. It has bar code scanner so plates created using Rock Maker and Formulator 16 can be read and either manually or automatically imaged at regular intervals.
We have also added Formulator 16 manufactured by Formulatrix which allows custom screen building and dispenses the screens using microfluidic technology. It can support 15+1 (water) ingredients and has a 96 nozzle dispensing the drops. Very low volumes of 200 nL to high volumes in few mL are achievable.
XRF User Fees
Starting in July 01, 2010, XRF has instituted user-fees to defray part of the cost of running the Facility. Please see XRF Fee Structure for more information about fees. New fees go into effect Jul 01, 2019.
XRF Boot Camp
Every August before the start of the Fall semester XRF runs a Boot Camp for new the graduate students. The three week Boot Camp is part of the Core Facilities Workshop. Please see XRF Boot Camp for this year’s schedule and syllabus.
Updated: June 20, 2019.