Young Scholars Program – 2014 Individual Research Project
- Protein Characterization: 2014 YSP Enzyme Characterization Introduction
- Protein Crystallization: 2014 YSP Primer on Crystallization and Crystal Mounting
The aim of the project is to crystallize a protein and study the effects differing relative humidity environment on crystal symmetry and dimensions. It will involve the following steps: 1) Specific laboratory skills in handling liquids, biological samples and liquid nitrogen. 2) Preparation of buffer solutions, enzyme samples and crystallization plates. 3) Setting-up crystal trays with enzyme samples. 4) Optical examination of crystal trays using a light microscope and recording results. 5) Mounting crystals in glass capillaries and equilibrating with salt solutions. 6) Examination of crystals using x-ray diffraction instrument and recording results. 7) Drawing conclusions based on the observations. It is expected that Steps 1-3 can be completed during the first three weeks, Steps 4 and 5 can be completed during the fourth week, Steps 6 and 7 during the fifth week leaving the last week for final analysis and presentation of the results.
2014 IRP Paper and Poster
2014 IRP Photos
Updated: November 24, 2015.