Young Scholars Program – 2010 Individual Research Project
This is my fourth year that I have been involved with Young Scholars Program (YSP) from Department of Biological Science since 2007 and sponsored Individual Research Project (IRP) each year. The following is the details of 2010 IRP.
The aim of the project is to crystallize a protein using different monovalent anions (like chloride, bromide, and iodide) and study the effect of the anions on crystal symmetry and dimensions. It is an extension of last year’s YSP work and will involve the following steps: 1) Specific laboratory skills in handling liquids, biological samples and liquid nitrogen. 2) Preparation of buffer solutions, enzyme samples and crystallization plates. 3) Setting-up crystal trays with enzyme samples and salt solutions. 4) Flash cooling of crystals in liquid nitrogen and storing of samples. 5) Examination of crystals using x-ray diffraction instrument and recording results. 6) Drawing conclusions based on the observations. It is expected that Steps 1-3 can be completed during the first three weeks, Steps 4 and 5 can be completed during the fourth week, Step 6 during the fifth week leaving the last week for final analysis and presentation of the results.
2010 IRP Paper, Poster, and Photos
2010 IRP Photos
Updated: November 24, 2015.