Young Scholars Program – 2008 Individual Research Project
This is my second year with Young Scholars Program (YSP) from Department of Biological Science since 2007 and sponsored Individual Research Project (IRP) each year. The following is the details of 2008 IRP.
The aim of the project is to crystallize a biological enzyme lysozyme with different sizes and study the effect of crystal sizes on cryo cooling rates and cell dimensions. This project is an extension of last year’s YSP work. The project will involve the following steps:
1) Specific laboratory skills in handling liquids, biological samples and liquid nitrogen. 2) Preparation of buffer solutions, enzyme samples and crystallization plates. 3) Setting-up crystal trays with enzyme samples and salt solutions. 4) Optical examination of crystal trays using a light microscope and recording results. 5) Flash cooling of crystals in liquid nitrogen and storing of samples. 6) Examination of crystals using x-ray diffraction instrument and recording results. 7) Drawing conclusions based on the observations. It is expected that Steps 1-3 can be completed during the first three weeks, Steps 4 and 5 can be completed during the fourth week, Steps 6 and 7 during the fifth week leaving the last week for final analysis and presentation of the results.
2008 IRP Paper and Poster
2008 IRP Photos
Updated: November 24, 2015.