Protein Crystallization and X-Ray Diffraction (pcxrd)

The practical course in crystallization and diffraction is intended to be a precursor to a graduate level macromolecular crystallization special topics course but the emphasis here will be more hands-on training and lab work than the graduate course.

2015 Course Schedule

  • Duration of the program: 3 h/session | 3 sessions/week | ~3 weeks total
  • Areas covered: Wet-lab (biochemistry), instrumentation (cryo and ambient crystallography), and computation (data processing and visualization)

2015 Syllabus

  • 1. Symmetry, lattices, and unit cell [presentation]  |  2. Crystals and protein crystallization set-up [hands-on]
  • 3. Crystallization observation and optimization [hands-on]   | 4. X-Rays, radiation safety and X-Ray generation [presentation]
  • 5. Cryogenics safety and cryo crystallography [hands-on]  | 6. X-Ray Diffraction set-up and data collection [hands-on]
  • 7. Data processing and visualization [hands-on] 8. Molecular graphics and data analysis [hands-on]

Resources

Photos

Updated: August 20, 2015.