Protein Crystallization and X-Ray Diffraction (pcxrd)
The practical course in crystallization and diffraction is intended to be a precursor to a graduate level macromolecular crystallization special topics course but the emphasis here will be more hands-on training and lab work than the graduate course.
- Duration of the program: 3 hour per session | 2 sessions per week | ~2 weeks total
- Number of days for lectures: 3 | Number of days for experiments: 4
- Areas covered: Wet-lab (biochemistry), instrumentation (crystallography), and computation (Linux and data processing)
- 1. Symmetry, lattices, and unit cell [presentation] | 2. Crystals and protein crystallization set-up [hands-on]
- 3. Crystallization observation and optimization [hands-on] | 4. X-Rays, radiation safety and X-Ray generation [presentation]
- 5. Cryogenics safety and cryo crystallography [hands-on] | 6. X-Ray Diffraction set-up and data collection [hands-on]
- 7. Linux operating system and data processing [presentation] | 8. Protein Data Bank and molecular graphics [hands-on]
Photos from 2014 XRF Boot Camp.
Updated: February 19, 2019.