Core Facilities Workshop: XRF Boot Camp

Protein Crystallization and X-Ray Diffraction (pcxrd)

The practical course in crystallization and diffraction is intended to be a precursor to the one semester graduate level macromolecular crystallization special topics course taught every other year. In this course, we will cover basic concepts of crystals, symmetry, crystallization, x-ray generation, radiation safety, x-ray diffraction, cryo safety, cryogenic technique, data collection, data processing, Linux o/s, computer graphics, data visualization, and model building. More emphasis will be given to hands-on training and lab work than the full-fledged graduate course.

2012 Course Schedule

At the end of this course, you will learn basic concepts in:

  • Protein crystallization and crystal symmetry
  • Cryogenic safety and cryo-crystallography
  • Need for crystals, cryogenic temperatures, and X-Rays
  • X-Ray generation and safety
  • X-Ray diffraction and data collection
  • Linux operating system and running programs
  • Computer graphics and model visualization

2012 Syllabus

  • 1. Symmetry, lattices, crystals, and crystallization
  • 2. Crystals and protein crystallization set-up
  • 3. Crystallization observation and optimization
  • 4. X-Rays, radiation safety and X-Ray generation
  • 5. Cryogenics safety and cryo-crystallography
  • 6. X-Ray Diffraction set-up and data collection
  • 7. Linux operating system and computer graphics
  • 8. Data processing and visualization and wrap-up

Sessions high-lighted in bold will involve more hands-on training time and sessions high-lighted in italics will involve more lecture time.

Updated: July 26, 2013.