Molecular Biophysics Graduate Student
Major Professor: Dr. P. Bryant Chase
Lab: 222 BIO (Biology Unit 1)
We have designed a FRET-based cardiac Troponin C sensor that responds to Ca2+ binding, using multiple FRET pair of fluorophores (Cyan/Yellow). The newly engineered protein was characterized in it is native state as well as with mutant Troponin C where one or more Ca2+ binding site was inactivated. This gives us information about cardiac troponin C,the ca sensing subunit in the cardiac muscle responsible for muscle contraction. Our construct could also be used as a geneticalle encoded Ca indicator, for calcium detection in cells, and possibly live cell imaging.
July 2007, Bachelor of Science in Biology, Minor Psychology, American University of Beirut, Beirut, Lebanon
2008-2009: MARTECH (Material Research and Technology) Fellowship (internal grant FSU)
2009-2010: MARTECH (Material Research and Technology) Fellowship (internal grant FSU)
Fall 2012: Dissertation Research Grant (Graduate School, FSU)
“Human cardiac troponin C undergoes global conformational changes in response to divalent cation binding: solution studies of fluorescent protein constructs by FRET and Analytical UltraCentrifugation“. Myriam A. Badr, Michael W. Davidson, P. Bryant Chase. Biophysical Society meeting, 2013.
“Slowed Dynamics of Thin Filament Regulatory Units Reduces Ca2+ Sensitivity of Cardiac Biomechanical Function”. Loong C., Kataoka A., Badr M.,Rogers J, Chase P.B. Cellular and Molecular Bioengineering, January 2013 (in Press)
“Tropomyosin flexural rigidity and single Ca2+ regulatory unit dynamics: implications for cooperative regulation of cardiac muscle contraction and cardiomyocyte hypertrophy”. Loong CK*, Badr MA*, Chase PB. Front Physiol. 2012;3:80. Epub 2012 Apr 4. (*equal contribution)