X-Ray Crystallography Facility

RRID:SCR_017922

As one of the FSU-wide Core Facilities, the multi-user macromolecular x-ray crystallography facility (XRF) at the Kasha Laboratory Building (KLB) in the Institute of Molecular Biophysics (IMB) provides training, expertise, and assistance for crystallizing and determining the three-dimensional structures of biological macromolecules and their complexes.  It is available to all the members of Structural Biology Program and others at FSU. XRF is located on the East side of the fourth floor of KLB (see the floor plan). More facility information is available at X-Ray Facility page.

R-Axis-Website

Instruments & Accessories

XRF has Rigaku RU-H2R & RU-H3R x-ray generators, Osmic confocal mirror systems, Rigaku R-Axis IV++ IP detector. It has Oxford CryoStream cryo coolersAmerican Magnetics auto-refill system stereo-zoom microscopes, crystallization chambers maintained at variety of temperatures, and data processing hardware and software. It has an Art Robbins Instruments Crystal Gryphon crystallization robot, Formulatrix Rock Imager 1 and Formulator 16, Rumed Crystal Incubator, and IMB designed, and home built simple robotic imager. The Facility has a ThorLabs DCC1645C digital camera for the Leica S8 APO microscope. More Info >>>

Recognition of Core Facility/Core Director Contributions

IMB Core Research Facilities are an integral part of research conducted in the Institute and the resulting publications. However, because the Facility personnel are not part of the individual lab groups, and much of their work is either behind the scenes or early in a given project, it is easy to forget to give proper attribution for their contribution to the research. An acknowledgement is appropriate when a Facility and/or its Director is used for routine data collection, sample preparation, general advice, etc. When the Facility Director is involved at the level of method development, data analysis and interpretation, or other significant contribution to the project, (s)he should be included as a coauthor of the publication. Use X-Ray Facility’s RRID: SCR_017922.

Synchrotron Beam Access

The Facility Director also coordinates both remote and mail-in synchrotron x-ray diffraction data collection work using FSU’s membership in NSLS-II Beamline AMX-17-ID-1 and FMX-17-ID-2 . More Info >>>

XRF Fee Structure

Starting from July 01, 2010, XRF charges a user-fee to defray part of the cost of running the Facility. Please see XRF Fee Structure for more information about fees. Updated user fees go into effect Jul 01, 2019.

XRF Boot Camp

Every August before the start of the Fall semester, XRF runs a Boot Camp for new the graduate students. The three-week Boot Camp is part of the Core Facilities Workshop. Please see XRF Boot Camp for this year’s schedule and syllabus.  The 2022 Boot Camp was held in mid-August 2022.

Updated: August 24, 2022.

XRF Info
 

Facility Director

Contact and Address
Ph: 1-850-644-6448
tsomasundaram@fsu.edu 
Inst Molecular Biophysics, KLB 414
91 Chieftan Way
Florida State University
Tallahassee, FL 32306-4380
Facility Information (RRID:SCR_017922) Established in 1993, the XRF has served over 200+ users, including 30+ PIs from 9 institutions and has helped generate multitudes of peer reviewed publications.

Acknowledgement An acknowledgement is appropriate when a Facility and/or its Director is used for routine data collection, sample preparation, general advice, etc. When the Facility Director is involved at the level of method development, data analysis and interpretation, or other significant contribution to the project, (s)he should be included as a coauthor of the publication.

Boot Camp For three week period in early August XRF runs a Core Facility Practical Training Course (Boot Camp) for all new graduate students. More information about 2022 Boot Camp is available here.