Protein Expression Facility Fee Structure
| Protein Expression Facility Fee Schedule (Effective: November 2014) | |
| Bacterial Expression | |
| E.coli expression trial, per construct | $80.50 |
| Protein expression and production from E.coli 2L culture | $117.50 |
| Protein expression and production from E.coli, additional 2L (up to 24L) | $12.00 |
| Cell pellet production, E.coli, per liter | $34.70 |
| Baculovirus Expression | |
| Insect cell pellet production, per liter | $169.50 |
| Baculovirus production per construct/50 ml | $5.25 |
| Protein production in insect cells from 1L | $206.50 |
| Mammalian expression | |
| Expression trial, per construct | $90.75 |
| Protein production in mammalian cell from 1L | $216.75 |
| Suspension culture protein expression, additional liter | $48.50 |
| Protein Purification | |
| Single step | $74.50 |
| Two steps | $127.00 |
| Three steps | $186.50 |
| Endotoxin removal | $100.00 |
| Tag cleavage with protease | $45.50 |
| Additional services | |
| Cell counter (Countess 2) | $2.50 |
| Competent cell (BL21, BL21(DE3),DH5alpha, Top10, Rosetta(DE3)
SoluBL21, XL10-gold, Nico21(DE3), BL21-Codonplus(DE3)-RIPL, DH10B, DH10Bac |
$0.65 |
| Proteases (Ulp1, 3C protease) | $2.63 |
| Enzymes (T7 RNA Polymerase) | $2.63 |
* For a cost estimate for your external project, please contact the facility director.