PBF Resources and Equipment
| Equipment Photos | Description |
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Beckman-Coulter Analytical Ultra-centrifuge: “The analytical ultracentrifuge is still the most versatile, rigorous and accurate means for determining the molecular weight and the hydrodynamic and thermodynamic properties of a protein or other macromolecule. No other technique is capable of providing the same range of information with a comparable level of precision and accuracy. The reason for this is that the method of sedimentation analysis is firmly based in thermodynamics.” Read more. |
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Circular Dichroism A circular dichroism (CD) spectrometer measures differential absorbance of left- and right-hand circularly polarized light, which provides the basis for the conformational analysis of macromolecules. Since the far-UV CD spectra are distinct for the respective secondary structure elements of proteins (such as a-helix and ß-sheet), the protein secondary structure can be determined. In addition, the CD spectral features in the near-UV region provide insights into the tertiary structure of proteins. CD spectroscopy is also employed in the structural studies of nucleic acids. More… |
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Size Exclusion-Multiangle Light Scattering (SECMALS), Wyatt Technologies |
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Stopped Flow Spectrometer, Applied PhotophysicsThe Applied Photophysics SX20 stopped-flow system monitors changes in fluorescence or absorbance by macromolecular solutions triggered by rapid alteration in buffer composition. As such, the technique is sensitive to changes in the conformational and dynamic characteristics of biomolecules and biomolecular complexes. The stopped-flow fluorescence measurements are typically used to determine rates of protein folding/unfolding and to monitor kinetic reactions on a millisecond time scale. |
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Cary Eclipse Spectrofluorimeter, The Varian Eclipse fluorescence spectrophotometer is computer controlled with fluorescence, phosphorescence, chemiluminescence and bioluminescence acquisition modes. The Biopack software controls the following applications: simple reads, spectral scanning, kinetics, anisotropy, lifetime and concentration determination.VendorVarian (Agilent Technologies) |
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Isothermal titration calorimetry (ITC) is used in thermodynamic analyses of macromolecular interactions. This technique is often referred to as a true in-solution method, as it does not require chemical tagging or immobilization of the interacting components. The measured heat, either absorbed or released upon binding, provides the basis for determination of thermodynamic parameters. More |
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Dynamic Light ScatteringDynamic Light Scattering (DLS) provides information about the size and distribution of macromolecules in solution. It allows for determination of the hydrodynamic radius, translational diffusion coefficient, polydispersity, and an estimated molecular weight of a macromolecule. More… |
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Cary 300 Bio UV/VisThe Cary 300 is a research-grade spectrophotometer whose peltier-controlled, multicell holder system is especially suited for enzyme kinetics and thermal denaturation experiments.The Cary 300 is controlled by the new Cary WinUV software. This Windows based software features a modular design which makes it easy to use. The instrument is shipped with liquid sample holders and of course it can be fitted with a wide range of accessories to provide extra capabilities |
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A whole range of small diagnostic tools. The PBF can be considered as a dry lab and therefore most sample preparations are done at the user’s lab. The PBF has however, small equipment used for measuring, mixing and routine analysis of samples. More… |
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